• Product name
  • Description
    Rabbit polyclonal to SMAD1/5/9
  • Host species
  • Specificity
    ab80255 detects endogenous levels of total SMAD1/5/9 protein.
  • Tested applications
    Suitable for: IHC-FoFr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from N terminal of human SMAD1/5/9

  • Positive control
    • HeLa cell extracts (WB), P7 rat cortex (IHC)



Our Abpromise guarantee covers the use of ab80255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/10000.


  • Relevance
    SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • AI528653 antibody
    • BSP1 antibody
    • DKFZp781C1895 antibody
    • DKFZp781O1323 antibody
    • Dwarfin A antibody
    • Dwf A antibody
    • Dwfc antibody
    • hSMAD1 antibody
    • hSmad5 antibody
    • JV4 1 antibody
    • JV41 antibody
    • JV5 1 antibody
    • MAD (mothers against decapentaplegic, Drosophila) homolog 1 antibody
    • MAD (mothers against decapentaplegic, Drosophila) homolog 9 antibody
    • MAD homolog 1 antibody
    • MAD homolog 9 antibody
    • MAD homolog1 (mothers against decapentaplegic, Drosophila) antibody
    • Mad related protein 1 antibody
    • MAD, mothers against decapentaplegic homolog 9 antibody
    • Madh1 antibody
    • MADH5 antibody
    • MADH6 antibody
    • MADH9 antibody
    • MADR1 antibody
    • Mlp1 antibody
    • Mothers against decapentaplegic, drosophila, homolog of, 9 antibody
    • Mothers against DPP homolog 1 antibody
    • Mothers against DPP homolog 9 antibody
    • MusMLP antibody
    • OTTHUMP00000178565 antibody
    • OTTMUSP00000022970 antibody
    • OTTMUSP00000022971 antibody
    • OTTMUSP00000022972 antibody
    • OTTMUSP0000002324 antibody
    • OTTMUSP00000023428 antibody
    • Sma- and Mad-related protein 1 antibody
    • SMAD antibody
    • SMAD family member 5 antibody
    • SMAD8 antibody
    • SMAD8A antibody
    • SMAD8B antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta signaling protein 1 antibody
    see all


  • All lanes : Anti-SMAD1/5/9 antibody (ab80255) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : HeLa cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Immunohistomchemical detection of SMAD1/5/9 using antibody ab80255 on perfusion fixed P7 rat cortex sections. The animals were perfused with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab80255, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview


This product has been referenced in:
  • Loscertales M  et al. Type IV collagen drives alveolar epithelial-endothelial association and the morphogenetic movements of septation. BMC Biol 14:59 (2016). IHC . Read more (PubMed: 27412481) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (embryonic stem cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
embryonic stem cells
Blocking step
Odyssey blocking buffer, LiCor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 01 2016

Thank you for contacting us.

We currently have two rabbit polyclonal antibodies that will detect SMAD 1/5:

Rabbit polyclonal to SMAD1/5/9 - ab80255
Rabbit polyclonal to SMAD 1/5/8/9 - ab13723

We have a number of antib...

Read More

Thank you for contacting us. Of the three antibodies we discussed yesterday, ab80255 is the best choice, given that we know the immunogen is derived from the N terminus, and your detection antibody is directed against the C terminus. The epitope that a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Cell (Rat cortex (day P7))
Rat cortex (day P7)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 21 2009


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