Anti-SMAD1/5/9 antibody (ab80255)
Key features and details
- Rabbit polyclonal to SMAD1/5/9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-SMAD1/5/9 antibody
See all SMAD1/5/9 primary antibodies -
Description
Rabbit polyclonal to SMAD1/5/9 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide derived from N terminal of human SMAD1/5/9
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab80255 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
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Relevance
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 4086 Human
- Entrez Gene: 4090 Human
- Entrez Gene: 4093 Human
- Entrez Gene: 17125 Mouse
- Entrez Gene: 17129 Mouse
- Entrez Gene: 55994 Mouse
- Omim: 601595 Human
- Omim: 603110 Human
see all -
Alternative names
- AI528653 antibody
- BSP1 antibody
- DKFZp781C1895 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab80255 has been referenced in 11 publications.
- Kabwe JC et al. CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension. Respir Res 23:87 (2022). PubMed: 35395852
- Nakajima T et al. Changes in Smad1/5/9 expression and phosphorylation in astrocytes of the rat hippocampus after transient global cerebral ischemia. J Chem Neuroanat 113:101941 (2021). PubMed: 33711423
- Jin T et al. CHI3L1 promotes Staphylococcus aureus-induced osteomyelitis by activating p38/MAPK and Smad signaling pathways. Exp Cell Res 403:112596 (2021). PubMed: 33826950
- Hong N et al. The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling. Cell Death Dis 12:393 (2021). PubMed: 33846290
- Liu L et al. A high level of lncFGD5-AS1 inhibits epithelial-to-Mesenchymal transition by regulating the miR-196a-5p/SMAD6/BMP axis in gastric Cancer. BMC Cancer 21:453 (2021). PubMed: 33892661