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Recombinant human protein purified from E.coli (His-Smad1)
Our Abpromise guarantee covers the use of ab75273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|WB||1/1000 - 1/2000. Predicted molecular weight: 52 kDa.|
|ELISA||Use at an assay dependent concentration.|