Overview

  • Product nameAnti-Smad2 + Smad3 antibody
    See all Smad2 + Smad3 primary antibodies
  • Description
    Rabbit polyclonal to Smad2 + Smad3
  • Specificityab63672 detects endogenous levels of total Smad2 and Smad3.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human Smad2 and Smad3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).

  • Positive control
    • COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab63672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa.

Target

  • RelevanceSMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • Cellular localizationCytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Database links
  • Alternative names
    • hMAD 2 antibody
    • hMAD 3 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • Mad related protein 2 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • Sma and Mad related protein 2 antibody
    • SMA and MAD related protein 3 antibody
    • SMAD 2 antibody
    • SMAD 3 antibody
    • SMAD family member 2 antibody
    • SMAD family member 3 antibody
    see all

Anti-Smad2 + Smad3 antibody images

  • All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution

    Lane 1 : COS7 cell extracts treated with UV (15mins)
    Lane 2 : COS7 cell extracts untreated
    Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins)
    Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-Smad2 + Smad3 antibody (ab63672)

This product has been referenced in:
  • Walshe TE  et al. TGF-ß signaling is required for maintenance of retinal ganglion cell differentiation and survival. Neuroscience 189:123-31 (2011). WB ; Rat . Read more (PubMed: 21664439) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"