Anti-Smad2 + Smad3 antibody (ab63672)
Key features and details
- Rabbit polyclonal to Smad2 + Smad3
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
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Overview
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Product name
Anti-Smad2 + Smad3 antibody
See all Smad2 + Smad3 primary antibodies -
Description
Rabbit polyclonal to Smad2 + Smad3 -
Host species
Rabbit -
Specificity
ab63672 detects endogenous levels of total Smad2 and Smad3. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse -
Immunogen
Synthetic non-phosphopeptide derived from human Smad2 and Smad3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 48 kDa.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 48 kDa. |
Target
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Relevance
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. -
Cellular localization
Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. -
Database links
- Entrez Gene: 4087 Human
- Entrez Gene: 4088 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 17127 Mouse
- Omim: 601366 Human
- Omim: 603109 Human
- SwissProt: P84022 Human
- SwissProt: Q15796 Human
see all -
Alternative names
- hMAD 2 antibody
- hMAD 3 antibody
- hSMAD2 antibody
see all
Images
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All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts treated with UV (15mins)
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts untreated
Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins)
Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Observed band size: 48 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab63672 has been referenced in 16 publications.
- Yamamoto A et al. Therapeutic Effect of Cyclin-Dependent Kinase 4/6 Inhibitor on Dermal Fibrosis in Murine Models of Systemic Sclerosis. Arthritis Rheumatol 74:860-870 (2022). PubMed: 34882985
- Liao CG et al. Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis. Oncogene 41:1780-1794 (2022). PubMed: 35132181
- Yin J et al. Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-ß1/Smad2/3 Signaling Pathway. Biomed Res Int 2020:3451215 (2020). PubMed: 32258113
- Zong M et al. Irbesartan ameliorates myocardial fibrosis in diabetic cardiomyopathy rats by inhibiting the TGFß1/Smad2/3 pathway. Exp Ther Med 20:117 (2020). PubMed: 33005243
- Wang T et al. A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells. Stem Cell Res Ther 11:365 (2020). PubMed: 32831139