• Product nameAnti-Smad2 + Smad3 antibodySee all Smad2 + Smad3 primary antibodies ...
  • Description
    Rabbit polyclonal to Smad2 + Smad3
  • Tested applicationsWB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to the N-terminal of human Smad2 + Smad3, identical to the related mouse and rat sequence.

  • Positive control
    • Human intestine cancer tissue, HeLa cell lysate



Our Abpromise guarantee covers the use of ab65847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 52 kDa (predicted molecular weight: 48, 52 kDa).
IHC-P Use a concentration of 1 - 2 µg/ml.
IHC-Fr Use a concentration of 0.4 - 1 µg/ml. Formalin or acetone fixed tissues.


  • RelevanceSMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • Cellular localizationCytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Database links
  • Alternative names
    • DKFZP586N0721 antibody
    • DKFZp686J10186 antibody
    • hMAD2 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • HSPC193 antibody
    • HsT17436 antibody
    • JV15 2 antibody
    • JV18 antibody
    • JV181 antibody
    • Mad mothers against decapentaplegic homolog 2 antibody
    • MAD mothers against decapentaplegic homolog 3 antibody
    • Mad3 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • MGC22139 antibody
    • MGC34440 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • SMA and MAD related protein 3 antibody
    see all

Anti-Smad2 + Smad3 antibody images

  • Anti-Smad2 + Smad3 antibody (ab65847) at 1 µg/ml + Hela cell lysate

    Predicted band size : 48, 52 kDa
    Observed band size : 48,52 kDa
  • ab65847 at 1 µg/ml staining Human intestine cancer tissue; paraffin embedded.

References for Anti-Smad2 + Smad3 antibody (ab65847)

This product has been referenced in:
  • Adhikari N  et al. Increase in GLUT1 in Smooth Muscle Alters Vascular Contractility and Increases Inflammation in Response to Vascular Injury. Arterioscler Thromb Vasc Biol : (2010). IHC-P ; Mouse . Read more (PubMed: 20947823) »
  • Wang H  et al. miR-106b aberrantly expressed in a double transgenic mouse model for Alzheimer's disease targets TGF-ß type II receptor. Brain Res 1357:166-74 (2010). Read more (PubMed: 20709030) »

See all 2 Publications for this product

Product Wall

We carry products which recognize mouse SMAD1/9, SMAD2/3 or SMAD1/5/9. There is no antibody currently on our catalog which recognizes all forms of SMAD in mouse. I have added links to our anti- SMAD1/9, SMAD2/3 and SMAD1/5/9 antibodies for your review ...

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Thank you for contacting us.

We do have a number of anti-SMAD2 and anti-SMAD3 antibodies which we guarantee in IHC-P on mouse. We to don't have the opportunity to test specific products against one another in each application but based on ...

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Thank you for your inquiry.

I am happy to confirm that antibodies are very stable structures.IgG antibodieshave a half-life of ca 35 days in the human body and that is at 37C and with ongoing degradation of the antibodies by mononuclearic pha...

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Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products
I can confirm that one vial of ab53100 with lot number is GR54038 has been added in order 1074322.
Thank you for your help and coo...

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Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I apprecia...

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