Overview

  • Product name
  • Description
    Goat polyclonal to Smad2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: SEIWGLSTPNTID, corresponding to Internal sequence amino acids 82-94 of Human MADH2.

  • General notes
    Principal Names: MADH2 (mothers against decapentaplegic homolog 2), JV18, MADR2, SMAD2, JV18-1. Official Gene Symbol: MADH2 GenBank Accession Number: NP005892 LocusLink ID: 4087

Properties

Applications

Our Abpromise guarantee covers the use of ab1305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 51 kDa in HeLa lysates (predicted molecular weight: 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
    • Tissue specificity
      Expressed at high levels in skeletal muscle, heart and placenta.
    • Sequence similarities
      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      modifications
      Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
      In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
      Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
    • Information by UniProt
    • Database links
    • Alternative names
      • Drosophila, homolog of, MADR2 antibody
      • hMAD-2 antibody
      • HsMAD2 antibody
      • JV18 antibody
      • JV18-1 antibody
      • JV181 antibody
      • MAD antibody
      • MAD homolog 2 antibody
      • MAD Related Protein 2 antibody
      • Mad-related protein 2 antibody
      • MADH2 antibody
      • MADR2 antibody
      • MGC22139 antibody
      • MGC34440 antibody
      • Mother against DPP homolog 2 antibody
      • Mothers against decapentaplegic homolog 2 antibody
      • Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody
      • Mothers against DPP homolog 2 antibody
      • OTTHUMP00000163489 antibody
      • Sma and Mad related protein 2 antibody
      • Sma- and Mad-related protein 2 MAD antibody
      • SMAD 2 antibody
      • SMAD family member 2 antibody
      • SMAD, mothers against DPP homolog 2 antibody
      • SMAD2 antibody
      • SMAD2_HUMAN antibody
      see all

    Images



    • Predicted band size : 51 kDa


      Smad2 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References

    ab1305 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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