• Product name
    Anti-Smad2 + Smad3 (phospho T8) antibody
    See all Smad2 + Smad3 primary antibodies
  • Description
    Rabbit polyclonal to Smad2 + Smad3 (phospho T8)
  • Specificity
    This antibody detects endogenous levels of Smad2 + Smad3 only when phosphorylated at threonine 8.
  • Tested applications
    Suitable for: IHC, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized phosphopeptide derived from human Smad2+Smad3 around the phosphorylation site of threonine 8 (PFTPPP)

  • Positive control
    • Extracts from RAW264.7 cells



Our Abpromise guarantee covers the use of ab63399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa.
ELISA 1/20000.


  • Relevance
    SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • Cellular localization
    Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Database links
  • Alternative names
    • hMAD 2 antibody
    • hMAD 3 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • Mad related protein 2 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • Sma and Mad related protein 2 antibody
    • SMA and MAD related protein 3 antibody
    • SMAD 2 antibody
    • SMAD 3 antibody
    • SMAD family member 2 antibody
    • SMAD family member 3 antibody
    • smad2+3 antibody
    • smad2/3 antibody
    see all


  • Embedded mouse lung sections stained for Phospho-smad2/3 (red) using ab63399 in immunohistochemical analysis (PMID: 28004808).

    Nucelar counterstain: DAPI (blue).
    TGF-β (green).

  • All lanes : Anti-Smad2 + Smad3 (phospho T8) antibody (ab63399) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells
    Lane 2 : Extracts from RAW264.7 cells plus phospho peptide

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.


This product has been referenced in:
  • Chen H  et al. Shenqiwan Ameliorates Renal Fibrosis in Rats by Inhibiting TGF-ß1/Smads Signaling Pathway. Evid Based Complement Alternat Med 2017:7187038 (2017). WB ; Rat . Read more (PubMed: 28638433) »
  • Ko JW  et al. Pine bark extract (Pycnogenol®) suppresses cigarette smoke-induced fibrotic response via transforming growth factor-ß1/Smad family member 2/3 signaling. Lab Anim Res 33:76-83 (2017). WB ; Mouse . Read more (PubMed: 28747971) »

See all 10 Publications for this product

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