Recombinant full length protein corresponding to Human Smad3.
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR314501 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab75512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP568Y] to Smad3 (ab40854).
For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit monoclonal [EP568Y] to Smad3 (ab40854) as Detection.
|ChIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.05 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 48 kDa).|
|ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"