A synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 213 (PMSPPA)
Our Abpromise guarantee covers the use of ab63403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
ab63403 staining Smad3 (phospho S213) in Human A549 cells by Immunocytochemistry. Cells were fixed using the HOPE method and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.