A synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 213 (PMSPPA)
Our Abpromise guarantee covers the use of ab63403 in the following tested applications.
|ICC||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
ab63403 staining Smad3 (phospho S213) in Human A549 cells by Immunocytochemistry. Cells were fixed using the HOPE method and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.