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Synthetic peptide: PSIRCSSpVSp, corresponding to amino acids 417-425 of Human Smad3
Our Abpromise guarantee covers the use of ab51451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution. PubMed: 20519446|
|WB||1/2000 - 1/20000. Predicted molecular weight: 48 kDa. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.|
|ELISA||1/25000 - 1/100000.|
|ICC/IF||Use at an assay dependent dilution.|
ab51451 at 2.5 µg/ml staining epidermal keratinocytes at 40X. This image shows strong nuclear staining with localization of the antibody as the precipated red siganl and a hematoxylin purple nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"