Overview

  • Product nameAnti-SMAD5 antibody
    See all SMAD5 primary antibodies
  • Description
    Mouse monoclonal to SMAD5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: KPLDICEFPF GSKQKEVCIN PYHYKRVESP VLPPVLVPRH NEFNPQHSLL VQFRNLSHNE PHMPQNATFP DSFHQPNN, corresponding to amino acids 105-182 of Human SMAD5

Properties

Applications

Our Abpromise guarantee covers the use of ab55484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 52 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781C1895 antibody
    • DKFZp781O1323 antibody
    • Dwfc antibody
    • hSmad5 antibody
    • JV5 1 antibody
    • JV5-1 antibody
    • MAD homolog 5 antibody
    • MAD, mothers against decapentaplegic homolog 5 antibody
    • MADH 5 antibody
    • MADH5 antibody
    • Mothers against decapentaplegic homolog 5 antibody
    • mothers against decapentaplegic, drosophila, homolog of, 5 antibody
    • Mothers against DPP homolog 5 antibody
    • MusMLP antibody
    • SMA and MAD related protein 5 antibody
    • SMAD 5 antibody
    • SMAD family member 5 antibody
    • SMAD, mothers against DPP homolog 5 antibody
    • Smad5 antibody
    • SMAD5_HUMAN antibody
    see all

Anti-SMAD5 antibody images



  • Predicted band size : 52 kDa
    SMAD5 antibody (ab55484) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55484 at 10 ug/ml staining smad5 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-SMAD5 antibody (ab55484)

ab55484 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"