Overview

  • Product nameAnti-SMAD6 antibody
    See all SMAD6 primary antibodies
  • Description
    Mouse monoclonal to SMAD6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: RDEYKPLDLS DSTLSYTETE ATNSLITAPG EFSDASMSPD ATKPSHWCSV AYWEHRTRVG RLYAVYDQAV SIFYDLPQGS GFCLGQLNLE QRSESVRRTR , corresponding to amino acids 285-385 of Human SMAD6

Properties

Applications

Our Abpromise guarantee covers the use of ab55485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionAntagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.
  • Tissue specificityUbiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated by BMP type 1 receptor kinase and by PRKX.
    Ubiquitinated by WWP1.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSMAD6 antibody
    • MAD homolog 6 antibody
    • MADH6 antibody
    • MADH7 antibody
    • Mothers against decapentaplegic homolog 6 antibody
    • Mothers against DPP homolog 6 antibody
    • SMAD 6 antibody
    • SMAD family member 6 antibody
    • SMAD mothers against DPP homolog 6 antibody
    • Smad6 antibody
    • SMAD6_HUMAN antibody
    see all

Anti-SMAD6 antibody images

  • Western blot against tagged recombinant protein immunogen using ab55485 SMAD6 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa.

References for Anti-SMAD6 antibody (ab55485)

ab55485 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing
Sample Human Cell lysate - whole cell (HUVEC (human umbilical cord venous endothelial cel)
Specification HUVEC (human umbilical cord venous endothelial cel
Treatment 0.1% FCS for 18h, stimulation with Bmp6 (100ng/ml) for 0-180 min
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mrs. Elke Maas

Verified customer

Submitted Oct 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"