Overview

  • Product nameAnti-SMAD6 antibody
    See all SMAD6 primary antibodies
  • Description
    Rabbit polyclonal to SMAD6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SMAD6.

    (Peptide available as ab66351.)

  • Positive control
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF7, SHSY-5Y and U2OS.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab63713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • FunctionAntagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.
  • Tissue specificityUbiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated by BMP type 1 receptor kinase and by PRKX.
    Ubiquitinated by WWP1.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSMAD6 antibody
    • MAD homolog 6 antibody
    • MADH6 antibody
    • MADH7 antibody
    • Mothers against decapentaplegic homolog 6 antibody
    • Mothers against DPP homolog 6 antibody
    • SMAD 6 antibody
    • SMAD family member 6 antibody
    • SMAD mothers against DPP homolog 6 antibody
    • Smad6 antibody
    • SMAD6_HUMAN antibody
    see all

Anti-SMAD6 antibody images

  • All lanes : Anti-SMAD6 antibody (ab63713) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 130 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SMAD6 antibody (ab63713)

This product has been referenced in:
  • Chen RY  et al. Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells. World J Gastroenterol 20:14895-903 (2014). WB ; Human . Read more (PubMed: 25356049) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Isolated Primary NK Cells)
Gel Running Conditions Reduced Denaturing
Loading amount 250000 cells
Treatment Rest, IL15, TGFB, IL12, IL18 for 24 hrs
Specification Isolated Primary NK Cells
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Sian Drake

Verified customer

Submitted May 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"