The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
FunctionAntagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.
Tissue specificityUbiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
Sequence similaritiesBelongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain.
Post-translational modificationsPhosphorylated by BMP type 1 receptor kinase and by PRKX. Ubiquitinated by WWP1.
Mothers against decapentaplegic homolog 6 antibody
Mothers against DPP homolog 6 antibody
SMAD 6 antibody
SMAD family member 6 antibody
SMAD mothers against DPP homolog 6 antibody
Anti-SMAD6 antibody images
Western blot - SMAD6 antibody (ab63713)
All lanes : Anti-SMAD6 antibody (ab63713) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 52 kDa Observed band size : 52 kDa Additional bands at : 130 kDa,70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-SMAD6 antibody (ab63713)
This product has been referenced in:
Chen RY et al. Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells. World J Gastroenterol20:14895-903 (2014).
Read more (PubMed: 25356049) »