Overview

  • Product name
  • Description
    Goat polyclonal to SMAD9
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Pig
  • Immunogen

    Synthetic peptide:

    HSEPLMPHNATYPD

    , corresponding to internal sequence amino acids 166-179 of Human SMAD9.

  • Positive control
    • Human kidney tissue and Human skin lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab115900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 52 kDa.
ELISA 1/64000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
  • Tissue specificity
    Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
  • Involvement in disease
    Pulmonary hypertension, primary, 2
  • Sequence similarities
    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
  • Cellular localization
    Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAD homolog 9 antibody
    • Madh6 antibody
    • Mothers against decapentaplegic antibody
    • Mothers against decapentaplegic homolog 9 antibody
    • Mothers against DPP homolog 9 antibody
    • SMAD 9 antibody
    • SMAD family member 9 antibody
    • SMAD, mothers against DPP homolog 9 (Drosophila) antibody
    • SMAD8A antibody
    • SMAD8B antibody
    • Smad9 antibody
    • SMAD9_HUMAN antibody
    see all

Images

  • ab115900, at 5 µg/ml, staining SMAD9 in formalin fixed, paraffin embedded Human kidney by Immunohistochemistry. Developed using biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-SMAD9 antibody (ab115900) at 0.3 µg/ml + Human skin lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa

References

ab115900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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