Overview

  • Product nameAnti-SMAD9 antibody
    See all SMAD9 primary antibodies
  • Description
    Goat polyclonal to SMAD9
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Pig
  • Immunogen

    Synthetic peptide:

    HSEPLMPHNATYPD

    , corresponding to internal sequence amino acids 166-179 of Human SMAD9.

  • Positive control
    • Human kidney tissue and Human skin lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 52 kDa.
ELISA 1/64000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
  • Tissue specificityExpressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
  • Involvement in diseasePulmonary hypertension, primary, 2
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
  • Cellular localizationCytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAD homolog 9 antibody
    • Madh6 antibody
    • Mothers against decapentaplegic antibody
    • Mothers against decapentaplegic homolog 9 antibody
    • Mothers against DPP homolog 9 antibody
    • SMAD 9 antibody
    • SMAD family member 9 antibody
    • SMAD, mothers against DPP homolog 9 (Drosophila) antibody
    • SMAD8A antibody
    • SMAD8B antibody
    • Smad9 antibody
    • SMAD9_HUMAN antibody
    see all

Anti-SMAD9 antibody images

  • ab115900, at 5 µg/ml, staining SMAD9 in formalin fixed, paraffin embedded Human kidney by Immunohistochemistry. Developed using biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-SMAD9 antibody (ab115900) at 0.3 µg/ml + Human skin lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa

References for Anti-SMAD9 antibody (ab115900)

ab115900 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"