Overview

  • Product nameAnti-SMAD9 antibody
    See all SMAD9 primary antibodies
  • Description
    Rabbit polyclonal to SMAD9
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 199-228 of Human SMAD9 (NP_005896.1, NP_001120689.1).

  • Positive control
    • A549 cell line lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 52 kDa.
ICC/IF 1/10 - 1/50.

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
  • Tissue specificityExpressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
  • Involvement in diseasePulmonary hypertension, primary, 2
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
  • Cellular localizationCytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAD homolog 9 antibody
    • Madh6 antibody
    • Mothers against decapentaplegic antibody
    • Mothers against decapentaplegic homolog 9 antibody
    • Mothers against DPP homolog 9 antibody
    • SMAD 9 antibody
    • SMAD family member 9 antibody
    • SMAD, mothers against DPP homolog 9 (Drosophila) antibody
    • SMAD8A antibody
    • SMAD8B antibody
    • Smad9 antibody
    • SMAD9_HUMAN antibody
    see all

Anti-SMAD9 antibody images

  • Immunofluorescence of MCF-7 cells labelling SMAD9 with ab124094. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab124094 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SMAD9 immunoreactivity is localized to the cytoplasm and nucleus.

  • Anti-SMAD9 antibody (ab124094) at 1/100 dilution + A549 cell line lysates at 35 µg

    Predicted band size : 52 kDa

References for Anti-SMAD9 antibody (ab124094)

ab124094 has not yet been referenced specifically in any publications.

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