Anti-SMAD9 antibody (ab96698)
Key features and details
- Rabbit polyclonal to SMAD9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMAD9 antibody
See all SMAD9 primary antibodies -
Description
Rabbit polyclonal to SMAD9 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish -
Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 166 and 353 of Human SMAD9 (AAH11559).
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Positive control
- NT2D1 whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
Target
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Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). -
Tissue specificity
Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney. -
Involvement in disease
Pulmonary hypertension, primary, 2 -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. -
Cellular localization
Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. - Information by UniProt
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Database links
- Entrez Gene: 4093 Human
- Entrez Gene: 55994 Mouse
- Entrez Gene: 85435 Rat
- Omim: 603295 Human
- SwissProt: O15198 Human
- SwissProt: Q9JIW5 Mouse
- SwissProt: O54835 Rat
- Unigene: 123119 Human
see all -
Alternative names
- MAD homolog 9 antibody
- Madh6 antibody
- Mothers against decapentaplegic antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab96698 has been referenced in 3 publications.
- Gao L et al. Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis. J Transl Med 19:37 (2021). PubMed: 33472665
- McDonald GLK et al. Pharmacological Manipulation of Early Zebrafish Skeletal Development Shows an Important Role for Smad9 in Control of Skeletal Progenitor Populations. Biomolecules 11:N/A (2021). PubMed: 33668680
- Gregson CL et al. A Rare Mutation in SMAD9 Associated With High Bone Mass Identifies the SMAD-Dependent BMP Signaling Pathway as a Potential Anabolic Target for Osteoporosis. J Bone Miner Res 35:92-105 (2020). PubMed: 31525280