Overview

  • Product name
    Anti-SMAGP antibody
  • Description
    Rabbit polyclonal to SMAGP
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 54-84 of Human SMAGP

  • Positive control
    • K562 cell line lysates; Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 11 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in epithelial cell-cell contacts. May play a role in tumor invasiveness and metastasis formation.
  • Tissue specificity
    Detected in breast, endometrium, colon and biliary tract. Detected in polarized epithelial structures characterized by cell-cell adhesion (at protein level).
  • Sequence similarities
    Belongs to the SMAGP family.
  • Post-translational
    modifications
    O-glycosylated. The O-glycan is modified with sialic acid residues.
  • Cellular localization
    Cell membrane. Cytoplasmic vesicle membrane. Predominantly on lateral parts of the membrane, at cell-cell epithelial junctions. Detected on cytoplasmic membranes in undifferentiated tumors.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSMAGP antibody
    • Smagp antibody
    • SMAGP_HUMAN antibody
    • Small cell adhesion glycoprotein antibody
    • small cell transmembrane and glycosylated protein antibody
    • Small transmembrane and glycosylated protein antibody
    see all

Images

  • Anti-SMAGP antibody (ab104059) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 11 kDa
  • Immunohistochemistry analysis of SMAGP expression in formalin fixed and paraffin embedded Human breast carcinoma tissue using 1/50 an104059 followed by a peroxidase conjugated secondary antibody and DAB staining.

References

ab104059 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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