1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay play a role in epithelial cell-cell contacts. May play a role in tumor invasiveness and metastasis formation.
Tissue specificityDetected in breast, endometrium, colon and biliary tract. Detected in polarized epithelial structures characterized by cell-cell adhesion (at protein level).
Sequence similaritiesBelongs to the SMAGP family.
Post-translational modificationsO-glycosylated. The O-glycan is modified with sialic acid residues.
Cellular localizationCell membrane. Cytoplasmic vesicle membrane. Predominantly on lateral parts of the membrane, at cell-cell epithelial junctions. Detected on cytoplasmic membranes in undifferentiated tumors.
Immunohistochemistry analysis of SMAGP expression in formalin fixed and paraffin embedded Human breast carcinoma tissue using 1/50 an104059 followed by a peroxidase conjugated secondary antibody and DAB staining.
References for Anti-SMAGP antibody (ab104059)
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