Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 0.2% Gelatin, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 64 kDa).

Target

  • RelevanceB5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. Some cellular antigens are associated with EEV and these vary with the cell type used to grow the virus. B5R consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, and for EEV formation.
  • Cellular localizationCell Membrane
  • Alternative names
    • B5R antibody
    • EEV type I membrane glycoprotein antibody
    • Smallpox antibody

Anti-Smallpox B5R antibody images

  • All lanes : Anti-Smallpox B5R antibody (ab53309) at 0.1 µg/ml

    Lane 1 : recombinant fusion protein containing amino acids 180-194
    Lane 2 : fusion partner only


    Predicted band size : 64 kDa
    Observed band size : 30,60 kDa (why is the actual band size different from the predicted?)

References for Anti-Smallpox B5R antibody (ab53309)

ab53309 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab53309.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"