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Overview

  • Product nameAnti-SMAP2 antibody
    See all SMAP2 primary antibodies
  • Description
    Rabbit polyclonal to SMAP2
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence, corresponding to C terminal amino acids 326-429 of Human SMAP2.

  • Positive control
    • Human lymph node tissue; A431 cells; RT 4, U 251 MG, Human Liver and Tonsil cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.

Target

  • FunctionGTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network.
  • Sequence similaritiesContains 1 Arf-GAP domain.
  • Cellular localizationCytoplasm. Detected in multiple foci throughout the cytoplasm and in juxtanuclear structures.
  • Information by UniProt
  • Database links
    • Alternative names
      • RP1-228H13.3 antibody
      • Small ArfGAP2 antibody
      • SMAP 1L antibody
      • Smap2 antibody
      • SMAP2_HUMAN antibody
      • Stromal membrane associated GTPase activating protein 2 antibody
      • Stromal membrane-associated protein 1-like antibody
      • Stromal membrane-associated protein 2 antibody
      see all

    Anti-SMAP2 antibody images

    • Western blot - Anti-SMAP2 antibody (ab121675)
      Western blot - Anti-SMAP2 antibody (ab121675)
      Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
      Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
    • Western blot - Anti-SMAP2 antibody (ab121675)
      Western blot - Anti-SMAP2 antibody (ab121675)
      All lanes : Anti-SMAP2 antibody (ab121675) at 1/250 dilution

      Lane 1 : RT 4 lysate
      Lane 2 : U 251 MG lysate
      Lane 3 : Human plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human tonsil lysate

      Developed using the ECL technique
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAP2 antibody (ab121675)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAP2 antibody (ab121675)
      ab121675 at 1/300 dilution staining SMAP2 in Paraffin-embedded Human lymph node tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-SMAP2 antibody (ab121675)
      Immunocytochemistry/ Immunofluorescence - Anti-SMAP2 antibody (ab121675)
      ab121675 at 4 µg/ml staining SMAP2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

    References for Anti-SMAP2 antibody (ab121675)

    ab121675 has not yet been referenced specifically in any publications.

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