Overview

  • Product name
    Anti-SMAP2 antibody
  • Description
    Rabbit polyclonal to SMAP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence, corresponding to C terminal amino acids 326-429 of Human SMAP2.

  • Positive control
    • Human lymph node tissue; A431 cells; RT 4, U 251 MG, Human Liver and Tonsil cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.

Target

  • Function
    GTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network.
  • Sequence similarities
    Contains 1 Arf-GAP domain.
  • Cellular localization
    Cytoplasm. Detected in multiple foci throughout the cytoplasm and in juxtanuclear structures.
  • Information by UniProt
  • Database links
    • Alternative names
      • RP1-228H13.3 antibody
      • Small ArfGAP2 antibody
      • SMAP 1L antibody
      • Smap2 antibody
      • SMAP2_HUMAN antibody
      • Stromal membrane associated GTPase activating protein 2 antibody
      • Stromal membrane-associated protein 1-like antibody
      • Stromal membrane-associated protein 2 antibody
      see all

    Images

    • Western blot - Anti-SMAP2 antibody (ab121675)
      Western blot - Anti-SMAP2 antibody (ab121675)
      Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
      Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
    • Western blot - Anti-SMAP2 antibody (ab121675)
      Western blot - Anti-SMAP2 antibody (ab121675)
      All lanes : Anti-SMAP2 antibody (ab121675) at 1/250 dilution

      Lane 1 : RT 4 lysate
      Lane 2 : U 251 MG lysate
      Lane 3 : Human plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human tonsil lysate

      Developed using the ECL technique.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAP2 antibody (ab121675)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAP2 antibody (ab121675)
      ab121675 at 1/300 dilution staining SMAP2 in Paraffin-embedded Human lymph node tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-SMAP2 antibody (ab121675)
      Immunocytochemistry/ Immunofluorescence - Anti-SMAP2 antibody (ab121675)
      ab121675 at 4 µg/ml staining SMAP2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

    References

    ab121675 has not yet been referenced specifically in any publications.

    Publishing research using ab121675? Please let us know so that we can cite the reference in this datasheet.

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