Overview

  • Product nameAnti-SMARCA1 antibody
    See all SMARCA1 primary antibodies
  • Description
    Rabbit polyclonal to SMARCA1
  • Tested applicationsSuitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1004 and 1054 of human SMARCA1 (NP_003060.2).

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).

Not recommended for blotting crude sources of SMARCA1. ab72287 can be used for blotting enriched sources (e.g. immunoprecipitated and exogenously expressed) of SMARCA1.

Target

  • RelevanceThe protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ATP dependent helicase SMARCA1 antibody
    • global transcription activator homologous sequence antibody
    • ISWI antibody
    • Nucleosome remodeling factor subunit SNF2L antibody
    • NURF140 antibody
    • Probable global transcription activator SNF2L1 antibody
    • SMARCA1 antibody
    • SNF2-like 1 antibody
    • SNF2L antibody
    • SNF2L1 antibody
    • SNF2LB antibody
    • SNF2LT antibody
    • sucrose nonfermenting 2-like protein 1 antibody
    • SWI antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin a1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 1 antibody
    • SWI2 antibody
    see all

Anti-SMARCA1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMARCA1 with ab72287 at 1/5000 (0.2µg/ml). Detection: DAB.
  • Detection of Human SMARCA1 by Immunoprecipitation from HeLa whole cell lysate, (1 mg for IP, 20% of IP loaded) using ab72287 at 3µg/mg lysate for IP (Lane 1) and 1µg/ml for subsequent WB. Lane 2 represents control IgG IP.

References for Anti-SMARCA1 antibody (ab72287)

ab72287 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"