Overview

  • Product name
    Anti-SMARCA1 antibody - ChIP Grade
    See all SMARCA1 primary antibodies
  • Description
    Rabbit polyclonal to SMARCA1 - ChIP Grade
  • Tested applications
    Suitable for: IP, WB, ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human SMARCA1. Note: the amino acid sequence is proprietary)(Peptide available as ab38108.)

  • Positive control
    • This antibody gave a positive signal in Hek293 Nuclear Lysate, Jurkat Nuclear Lysate and SHSY5Y whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 123 kDa).

Abcam recommends using 3% milk as the blocking agent.

ICC/IF Use a concentration of 1 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 20452320

Target

  • Relevance
    The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ATP dependent helicase SMARCA1 antibody
    • global transcription activator homologous sequence antibody
    • ISWI antibody
    • Nucleosome remodeling factor subunit SNF2L antibody
    • NURF140 antibody
    • Probable global transcription activator SNF2L1 antibody
    • SMARCA1 antibody
    • SNF2-like 1 antibody
    • SNF2L antibody
    • SNF2L1 antibody
    • SNF2LB antibody
    • SNF2LT antibody
    • sucrose nonfermenting 2-like protein 1 antibody
    • SWI antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin a1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 1 antibody
    • SWI2 antibody
    see all

Images

  • All lanes : Anti-SMARCA1 antibody - ChIP Grade (ab37003) at 1 µg/ml (blocked with 3% milk)

    Lane 1 : Jurkat nuclear extract lysate (ab14844)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Nuclear Lysate
    Lane 3 : SHSY5Y Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 123 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab37003 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab37003, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • SMARCA1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to SMARCA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab37003.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 105kDa: SMARCA1.

References

This product has been referenced in:
  • Ye Z  et al. Genome-wide analysis reveals positional-nucleosome-oriented binding pattern of pioneer factor FOXA1. Nucleic Acids Res 44:7540-54 (2016). CHIPseq ; Human . Read more (PubMed: 27458208) »
  • Alvarez-Saavedra M  et al. Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. Nat Commun 5:4181 (2014). IHC-FrFl . Read more (PubMed: 24946904) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (Skin Fibroblasts)
Specification
Skin Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 07 2013

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