Anti-SMARCA1 antibody - ChIP Grade (ab49126)

Overview

  • Product nameAnti-SMARCA1 antibody - ChIP Grade
    See all SMARCA1 primary antibodies
  • Description
    Rabbit polyclonal to SMARCA1 - ChIP Grade
  • SpecificityThis antibody recognizes SMARCA1
  • Tested applicationsSuitable for: ChIP, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    A region within synthetic peptide: GAAAAATEGT TATEKGEKKE KITSPFQLKL AAKASKSEKE MDPEYEEKMV, corresponding to N terminal amino acids 38-87 of Mouse SMARCA1

  • Positive control
    • NIH3T3 lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceThe protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ATP dependent helicase SMARCA1 antibody
    • global transcription activator homologous sequence antibody
    • ISWI antibody
    • Nucleosome remodeling factor subunit SNF2L antibody
    • NURF140 antibody
    • Probable global transcription activator SNF2L1 antibody
    • SMARCA1 antibody
    • SNF2-like 1 antibody
    • SNF2L antibody
    • SNF2L1 antibody
    • SNF2LB antibody
    • SNF2LT antibody
    • sucrose nonfermenting 2-like protein 1 antibody
    • SWI antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin a1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 1 antibody
    • SWI2 antibody
    see all

Anti-SMARCA1 antibody - ChIP Grade images

  • ChIP analysis using ab49126 (0.5µg/mL) binding SMARCA1 in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.

  • Anti-SMARCA1 antibody - ChIP Grade (ab49126) at 1.25 µg/ml + NIH3T3 lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%

References for Anti-SMARCA1 antibody - ChIP Grade (ab49126)

ab49126 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I can confirm that the blocking solution is 5% skimmed milk in PBS. The antibody diluent is also 5% skimmed milk. Lastly, the incubation condition is 2 hours at 37℃. Of course, overnight at 4℃ is also ok for blotting. ...

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Thank you for your enquiry. I have contacted the originator of the product for more information. According to them, the immunogen sequence used to generate this antibody can also hit a 43kD isoform of SMARCA1 (as we observed in our laboratory) ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"