Overview

  • Product name
    Anti-SMARCA1 antibody [EPR12191]
    See all SMARCA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12191] to SMARCA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMARCA1 aa 1000 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P28370

  • Positive control
    • HeLa, 293T, SH-SY5Y and SK-OV-3 cell lysate; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Relevance
      The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • ATP dependent helicase SMARCA1 antibody
      • global transcription activator homologous sequence antibody
      • ISWI antibody
      • Nucleosome remodeling factor subunit SNF2L antibody
      • NURF140 antibody
      • Probable global transcription activator SNF2L1 antibody
      • SMARCA1 antibody
      • SNF2-like 1 antibody
      • SNF2L antibody
      • SNF2L1 antibody
      • SNF2LB antibody
      • SNF2LT antibody
      • sucrose nonfermenting 2-like protein 1 antibody
      • SWI antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin a1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 1 antibody
      • SWI2 antibody
      see all

    Images

    • All lanes : Anti-SMARCA1 antibody [EPR12191] (ab172483) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : SK-OV-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 123 kDa

    • Flow cytometric analysis of permeabilized 293T cells labeling SMARCA1 with ab172483 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Alvarez-Saavedra M  et al. Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. Nat Commun 5:4181 (2014). ChIP . Read more (PubMed: 24946904) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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