Recombinant Anti-SNF2L antibody [EPR12191] (ab172483)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12191] to SNF2L
- Suitable for: IP, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SNF2L antibody [EPR12191]
See all SNF2L primary antibodies -
Description
Rabbit monoclonal [EPR12191] to SNF2L -
Host species
Rabbit -
Specificity
Flow Cyt (Intra) and IP results based on Human reactivity.
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Tested applications
Suitable for: IP, WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6, RAW264,7, PC-12, NIH/3T3, HeLa, 293T, SH-SY5Y and SK-OV03 whole cell lysates. IP: RAW264.7 whole cell lysate. Flow Cyt: 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12191 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/30.
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WB |
1/1000. Predicted molecular weight: 123 kDa.
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Flow Cyt (Intra) |
1/10.
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Notes |
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IP
1/30. |
WB
1/1000. Predicted molecular weight: 123 kDa. |
Flow Cyt (Intra)
1/10. |
Target
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Relevance
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6594 Human
- Entrez Gene: 93761 Mouse
- Entrez Gene: 317575 Rat
- Omim: 300012 Human
- SwissProt: P28370 Human
- SwissProt: Q6PGB8 Mouse
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Alternative names
- ATP dependent helicase SMARCA1 antibody
- global transcription activator homologous sequence antibody
- ISWI antibody
see all
Images
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All lanes : Anti-SNF2L antibody [EPR12191] (ab172483) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 123 kDa
Observed band size: 123 kDa
Exposure time: 81 secondsBlocking buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-SNF2L antibody [EPR12191] (ab172483) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : SK-OV-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDa -
Blocking buffer and concentration: 5% NFDM/TBST.
All lanes : Anti-SNF2L antibody [EPR12191] (ab172483) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg
Lane 2 : RAW264.7 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab172483 in RAW264.7 whole cell lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 123 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Intracellular flow cytometric analysis of permeabilized 293Tcells labeling SNF2L with ab172483at 1/10 dilution (red)or a Rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab172483 has been referenced in 1 publication.
- Alvarez-Saavedra M et al. Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. Nat Commun 5:4181 (2014). ChIP . PubMed: 24946904