• Product nameAnti-SMARCA2 / BRM antibody
    See all SMARCA2 / BRM primary antibodies
  • Description
    Rabbit polyclonal to SMARCA2 / BRM
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 1354-1370 of SMARCA2 conjugated to maleimide-activated KLH via an N-terminal added cysteine.

  • Positive control
    • WB: Nuclear extracts of SMARCA2 transfected 293T cell line. ICC: Paraformaldehyde/Triton fixed SMARCA2 transfected 293T cell line.
  • General notesStorage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.



Our Abpromise guarantee covers the use of ab12165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIF(ICC): Use at a concentration of 1 µg/ml on paraformaldehyde/Triton fixed SMARCA2-transfected 293T cell line). Indirect IF.
    WB: Use at a concentration of 0.15 µg/ml (determined using nuclear
    extracts of hBrm-transfected 293T cells). Predicted molecular weight: 230 kDa. Staining of SMARCA2 is specifically inhibited by the immunizing peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
    • Involvement in diseaseNicolaides-Baraitser syndrome
    • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
      Contains 1 bromo domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HSA domain.
      Contains 1 QLQ domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent helicase SMARCA2 antibody
      • ATP-dependent helicase SMARCA2 antibody
      • BAF190 antibody
      • BAF190B antibody
      • BRG1-associated factor 190B antibody
      • BRM antibody
      • FLJ36757 antibody
      • Global transcription activator homologous sequence antibody
      • hBRM antibody
      • hSNF2a antibody
      • MGC74511 antibody
      • Possible global transcription activator SNF2L2 antibody
      • Probable global transcription activator SNF2L2 antibody
      • Protein brahma homolog antibody
      • SMARCA2 antibody
      • SMCA2_HUMAN antibody
      • SNF2 alpha antibody
      • SNF2 like 2 antibody
      • SNF2-alpha antibody
      • SNF2/SWI2 like protein 2 antibody
      • SNF2L2 antibody
      • SNF2LA antibody
      • Sth1p antibody
      • Sucrose nonfermenting 2 like protein 2 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 antibody
      see all

    References for Anti-SMARCA2 / BRM antibody (ab12165)

    This product has been referenced in:
    • Nan X  et al. Interaction between chromatin proteins MECP2 and ATRX is disrupted by mutations that cause inherited mental retardation. Proc Natl Acad Sci U S A 104:2709-14 (2007). WB ; Mouse . Read more (PubMed: 17296936) »

    See 1 Publication for this product

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