The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIF(ICC): Use at a concentration of 1 µg/ml on paraformaldehyde/Triton fixed SMARCA2-transfected 293T cell line). Indirect IF.
WB: Use at a concentration of 0.15 µg/ml (determined using nuclear
extracts of hBrm-transfected 293T cells). Predicted molecular weight: 230 kDa. Staining of SMARCA2 is specifically inhibited by the immunizing peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Involvement in diseaseNicolaides-Baraitser syndrome
Global transcription activator homologous sequence antibody
Possible global transcription activator SNF2L2 antibody
Probable global transcription activator SNF2L2 antibody
Protein brahma homolog antibody
SNF2 alpha antibody
SNF2 like 2 antibody
SNF2/SWI2 like protein 2 antibody
Sucrose nonfermenting 2 like protein 2 antibody
SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 antibody
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 antibody
References for Anti-SMARCA2 / BRM antibody (ab12165)
This product has been referenced in:
Nan X et al. Interaction between chromatin proteins MECP2 and ATRX is disrupted by mutations that cause inherited mental retardation. Proc Natl Acad Sci U S A104:2709-14 (2007).
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