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Synthetic peptide (Human) - which represents a portion of human Helicase Lymphoid Specific encoded in part by exons 15 and 16.
DNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination, and transcription. Jarvis et al. (1996) identified a mouse gene encoding a novel putative helicase. They designated the gene LSH, for lymphoid-specific Hel, because Northern blot analysis revealed that it was expressed specifically in mouse early thymocytes. Geiman et al. (1998) determined that the mouse LSH, or HELLS (helicase lymphoid-specific), gene contains at least 18 exons and spans more than 26.6 kb. Using Northern blot analysis, these authors found that human HELLS is expressed in testis and thymus, two tissues that are highly active in DNA recombination. Geiman and Muegge (2000) demonstrated that LSH is not obligatory for normal lymphoid development but is essential for normal proliferation of peripheral T lymphocytes.
Our Abpromise guarantee covers the use of ab3851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).|
|IP||Use a concentration of 2 - 4 µg/ml.|
Detection of Human HELLS by Western Blot of Immunoprecipitate using Affinity Purified Rabbit anti-HELLS (ab3851).
Sample: Nuclear Extract (1mg) from HeLa cells
Antibody: Affinity purified Rabbit anti-HELLS (ab3851)
IP - 2
Detection: ECL, 30 seconds
ab3851 has not yet been referenced specifically in any publications.
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