Anti-SMARCA6 antibody (ab3851)

Overview

  • Product nameAnti-SMARCA6 antibody
    See all SMARCA6 primary antibodies
  • Description
    Rabbit polyclonal to SMARCA6
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) - which represents a portion of human Helicase Lymphoid Specific encoded in part by exons 15 and 16.

  • Positive control
    • Nuclear extract from HeLa cells.
  • General notes


    DNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination, and transcription. Jarvis et al. (1996) identified a mouse gene encoding a novel putative helicase. They designated the gene LSH, for lymphoid-specific Hel, because Northern blot analysis revealed that it was expressed specifically in mouse early thymocytes. Geiman et al. (1998) determined that the mouse LSH, or HELLS (helicase lymphoid-specific), gene contains at least 18 exons and spans more than 26.6 kb. Using Northern blot analysis, these authors found that human HELLS is expressed in testis and thymus, two tissues that are highly active in DNA recombination. Geiman and Muegge (2000) demonstrated that LSH is not obligatory for normal lymphoid development but is essential for normal proliferation of peripheral T lymphocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesDNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination, and transcription. Jarvis et al. (1996) identified a mouse gene encoding a novel putative helicase. They designated the gene LSH, for lymphoid-specific Hel, because Northern blot analysis revealed that it was expressed specifically in mouse early thymocytes. Geiman et al. (1998) determined that the mouse LSH, or HELLS (helicase lymphoid-specific), gene contains at least 18 exons and spans more than 26.6 kb. Using Northern blot analysis, these authors found that human HELLS is expressed in testis and thymus, two tissues that are highly active in DNA recombination. Geiman and Muegge (2000) demonstrated that LSH is not obligatory for normal lymphoid development but is essential for normal proliferation of peripheral T lymphocytes.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP Use a concentration of 2 - 4 µg/ml.

Target

  • FunctionPlays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.
  • Tissue specificityHighly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus. Closely associated with pericentric heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10339 antibody
    • Helicase lymphoid specific antibody
    • Hells antibody
    • HELLS_HUMAN antibody
    • LSH antibody
    • Lymphoid specific helicase antibody
    • Lymphoid-specific helicase antibody
    • Nbla10143 antibody
    • PASG antibody
    • Proliferation associated SNF2 like protein antibody
    • Proliferation-associated SNF2-like protein antibody
    • SWI/SNF2 related matrix associated actin dependent regulator of chromatin subfamily A member 6 antibody
    • SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6 antibody
    see all

Anti-SMARCA6 antibody images

  • Detection of Human HELLS by Western Blot of Immunoprecipitate using Affinity Purified Rabbit anti-HELLS (ab3851).

    Sample: Nuclear Extract (1mg) from HeLa cells

    Antibody: Affinity purified Rabbit anti-HELLS (ab3851)

    IP - 2µg. WB - 0.5µg/ml

    Detection: ECL, 30 seconds

     

  • Anti-SMARCA6 antibody (ab3851) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 102 kDa,120 kDa,47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-SMARCA6 antibody (ab3851)

ab3851 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details. I am sorry to hear that these antibodies did not work in ICC/IF.
As requested, I have issued a free of charge replacement of ab3851, ab52060, and ab112511. These will be shipped out tonight for delivery tomor...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7 and HEK)
Loading amount 30 µg
Specification MCF7 and HEK
Gel Running Conditions Reduced Denaturing (8-16%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Aug 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Oocytes)
Loading amount 100 cells
Specification Oocytes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Aug 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Spermatogenic cells)
Specification Spermatogenic cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.15% Triton X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
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Verified customer

Submitted Aug 29 2007

I'm sorry to hear you are having a problem with ab3851. Thank you for taking the time to answer our questions and sending us an image of your blot, it is very helpful. I suspect the high background you had in the first blot is due to the presence of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"