SMARCA6 overexpression 293T lysate (whole cell) (ab94327)

Overview

  • Product nameSMARCA6 overexpression 293T lysate (whole cell)
  • Description
    SMARCA6 overexpression lysate
  • General notesab94327 is a 293T cell transfected lysate in which Human SMARCA6 has been transiently over-expressed using a pCMV-SMARCA6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis. Tissue specificity: Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias. Similarity: Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Applications

Our Abpromise guarantee covers the use of ab94327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

SMARCA6 overexpression 293T lysate (whole cell) images

  • ab94327 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SMARCA6 antibody (ab55095) at 1/500 dilution

    Lane 1 : SMARCA6 overexpression 293T lysate (whole cell) (ab94327)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for SMARCA6 overexpression 293T lysate (whole cell) (ab94327)

ab94327 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"