Overview

  • Product nameAnti-SMARCAD1 antibody
    See all SMARCAD1 primary antibodies
  • Description
    Mouse polyclonal to SMARCAD1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length SMARCAD1 protein (Human)

  • Positive control
    • SMARCAD1 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).
ICC/IF 1/250.

Target

  • FunctionProbable ATP-dependent DNA helicase.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 2 CUE domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase 1 antibody
    • ATP-dependent helicase 1 antibody
    • ETL1 antibody
    • hHEL1 antibody
    • Smarcad1 antibody
    • SMRCD_HUMAN antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 antibody
    see all

Anti-SMARCAD1 antibody images

  • All lanes : Anti-SMARCAD1 antibody (ab67548) at 1/500 dilution

    Lane 1 : SMARCAD1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 117 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • 4-cell stage mouse embryos were recovered and the zona pellucida removed. ab67548 at 1:250 dilution was used to detect SMARCAD1, and as secondary antibldy embryos were incubated in Alexa-fluor488 conjugated donkey anti-mouse IgG. Embryos were then imaged by fluorescence confocal microscopy and projected confocal stack images generated - as shown in two examples. Please see accompanying abreview for additional information.

    See Abreview

References for Anti-SMARCAD1 antibody (ab67548)

ab67548 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Pre-implantation mouse embryos (4 cell stage))
Specification Pre-implantation mouse embryos (4 cell stage)
Fixative Paraformaldehyde
Permeabilization Yes - 0.5 TritonX-100
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Dr. Alex Bruce

Verified customer

Submitted Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"