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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Purity Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).
Function Probable ATP-dependent DNA helicase.
Tissue specificity Ubiquitous.
Sequence similarities Belongs to the SNF2/RAD54 helicase family. Contains 2 CUE domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Nucleus.
Information by UniProt
ATP dependent helicase 1 antibody
ATP-dependent helicase 1 antibody
Anti-SMARCAD1 antibody images
Western blot - SMARCAD1 antibody (ab67548)
All lanes : Anti-SMARCAD1 antibody (ab67548) at 1/500 dilution Lane 1 : SMARCAD1 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Predicted band size : 117 kDa Observed band size : 90 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - SMARCAD1 antibody (ab67548) This image is courtesy of an Abreview submitted by Dr Alex Bruce
4-cell stage mouse embryos were recovered and the zona pellucida removed. ab67548 at 1:250 dilution was used to detect SMARCAD1, and as secondary antibldy embryos were incubated in Alexa-fluor488 conjugated donkey anti-mouse IgG. Embryos were then imaged by fluorescence confocal microscopy and projected confocal stack images generated - as shown in two examples. Please see accompanying abreview for additional information.
References for Anti-SMARCAD1 antibody (ab67548)
has not yet been referenced specifically in any publications.
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