Overview

  • Product name
  • Description
    Rabbit polyclonal to SmarcAL1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 120 and 457 of Human SmarcAL1 (UniProt ID: Q9NZC9)

  • Positive control
    • A549 whole cell lysate, A549 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA.
  • Tissue specificity
    Ubiquitously expressed, with high levels in testis.
  • Involvement in disease
    Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
    Contains 2 HARP domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HARP antibody
    • HepA Related Protein antibody
    • HepA-related protein antibody
    • hHARP antibody
    • SIOD antibody
    • SMAL1_HUMAN antibody
    • SMARCA like Protein 1 antibody
    • smarcal1 antibody
    • Sucrose nonfermenting protein 2 like 1 antibody
    • Sucrose nonfermenting protein 2-like 1 antibody
    • SWI/SNF Related antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A like protein 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 antibody
    see all

Images

  • Anti-SmarcAL1 antibody (ab154226) at 1/500 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 106 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SmarcAL1 with ab154226 at a 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image).

References

This product has been referenced in:
  • Keka IS  et al. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining. Nucleic Acids Res N/A:N/A (2015). Human . Read more (PubMed: 26089390) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
2e+006 cells
Immuno-precipitation step
Protein G
Specification
HeLa
Username

Abcam user community

Verified customer

Submitted Jan 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (Hek 293T)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
1e+007 cells
Specification
Hek 293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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