Overview

  • Product nameAnti-SmarcAL1 antibody
    See all SmarcAL1 primary antibodies
  • Description
    Rabbit polyclonal to SmarcAL1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: FDPGSASGTS GSSSQNMGDT LDESSLTASP QKKRRFEFFD NWDSFTSPL, corresponding to C terminal amino acids 906-954 of Human SmarcAL1

  • Positive control
    • 721 B lymphoblast cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA.
  • Tissue specificityUbiquitously expressed, with high levels in testis.
  • Involvement in diseaseDefects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
    Contains 2 HARP domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HARP antibody
    • HepA Related Protein antibody
    • HepA-related protein antibody
    • hHARP antibody
    • SIOD antibody
    • SMAL1_HUMAN antibody
    • SMARCA like Protein 1 antibody
    • smarcal1 antibody
    • Sucrose nonfermenting protein 2 like 1 antibody
    • Sucrose nonfermenting protein 2-like 1 antibody
    • SWI/SNF Related antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A like protein 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 antibody
    see all

Anti-SmarcAL1 antibody images

  • Anti-SmarcAL1 antibody (ab69900) at 1.25 µg/ml + 721 B lymphoblast cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 106 kDa
    Observed band size : 106 kDa

References for Anti-SmarcAL1 antibody (ab69900)

This product has been referenced in:
  • Postow L  et al. Identification of SMARCAL1 as a component of the DNA damage response. J Biol Chem 284:35951-61 (2009). WB ; Human . Read more (PubMed: 19841479) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 1e+007 cells
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Oct 21 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Permeabilization Yes - PBS1X + 0.5 % Triton X-100
Specification U2OS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

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Verified customer

Submitted Oct 03 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"