Overview

  • Product name
  • Description
    Rabbit polyclonal to SMARCC1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 808-1052 of Human SMARCC1 (Q92922).

  • Positive control
    • Rat RT2 xenograft tissue, Mouse C2C12 xenograft tissue, Human BT483 xenograft tissue; HeLa cells; NT2D1, IMR32 and U-87 MG whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab126180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificity
    Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • Post-translational
    modifications
    Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI115498 antibody
    • BAF 155 antibody
    • BAF155 antibody
    • BRG 1 associated factor 155 antibody
    • BRG1 associated factor 155 antibody
    • BRG1-associated factor 155 antibody
    • Chromatin remodeling complex BAF155 subunit antibody
    • CRACC 1 antibody
    • CRACC1 antibody
    • Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody
    • Rsc 8 antibody
    • Rsc8 antibody
    • SMARC C1 antibody
    • SMARCC 1 antibody
    • SMARCC1 antibody
    • SMRC1_HUMAN antibody
    • SRG 3 antibody
    • SRG3 antibody
    • SWI 3 antibody
    • SWI/SNF complex 155 kDa subunit antibody
    • SWI/SNF complex subunit SMARCC1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody
    • SWI3 antibody
    see all

Images

  • All lanes : Anti-SMARCC1 antibody (ab126180) at 1/5000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : IMR32 whole cell lysate
    Lane 3 : U-87 MG whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 123 kDa
  • ab126180, at a 1/500 dilution, staining SMARCC1 in paraformaldehyde-fixed HeLa cells by Immunofluorescence (green). Alpha tubulin filaments are stained in red and the nucleus in blue.
  • ab126180, at 1/500 dilution, staining SMARCC1 in paraffin-embedded Mouse C2C12 xenograft tissue by Immunohistochemistry.
  • ab126180, at 1/500 dilution, staining SMARCC1 in paraffin-embedded Rat RT2 xenograft tissue by Immunohistochemistry.
  • ab126180, at 1/500 dilution, staining SMARCC1 in paraffin-embedded Human BT483 xenograft tissue by Immunohistochemistry.

References

ab126180 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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