• Product nameAnti-SMARCC1 antibody
    See all SMARCC1 primary antibodies
  • Description
    Rabbit polyclonal to SMARCC1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human SMARCC1.

    (Peptide available as ab24351.)

  • Positive control
    • This antibody gave a positive signal for Western Blot in HeLa whole cell lysate. This antibody gave a positive signal for Immunofluorescence in HeLa cells.


Associated products


Our Abpromise guarantee covers the use of ab22355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 122 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificityExpressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • Post-translational
    Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI115498 antibody
    • BAF 155 antibody
    • BAF155 antibody
    • BRG 1 associated factor 155 antibody
    • BRG1 associated factor 155 antibody
    • BRG1-associated factor 155 antibody
    • Chromatin remodeling complex BAF155 subunit antibody
    • CRACC 1 antibody
    • CRACC1 antibody
    • Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody
    • Rsc 8 antibody
    • Rsc8 antibody
    • SMARC C1 antibody
    • SMARCC 1 antibody
    • SMARCC1 antibody
    • SMRC1_HUMAN antibody
    • SRG 3 antibody
    • SRG3 antibody
    • SWI 3 antibody
    • SWI/SNF complex 155 kDa subunit antibody
    • SWI/SNF complex subunit SMARCC1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody
    • SWI3 antibody
    see all

Anti-SMARCC1 antibody images

  • All lanes : Anti-SMARCC1 antibody (ab22355) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 ug
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 ug with Human SMARCC1 peptide (ab24351) at 1 µg/ml

    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Predicted band size : 122 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa (possible cleavage fragment,cross reactivity),40 kDa (possible cleavage fragment,cross reactivity),85 kDa (possible cleavage fragment,cross reactivity).
  • ICC/IF image of ab22355 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab22355, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas

    ab22355 staining SMARCC1in human kidney. Paraffin embedded human kidney tissue was incubated with ab22355 (1/2000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab22355 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

References for Anti-SMARCC1 antibody (ab22355)

ab22355 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Dr. Efrat Shema

Verified customer

Submitted Sep 07 2011