Overview

  • Product name
  • Description
    Rabbit polyclonal to SMARCD1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 250-300 of human SMARCD1 (NP_003067.2; GeneID 6602).

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Involved in chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • Tissue specificity
      Expressed in all tissues tested, including brain, heart, kidney, liver, lung, muscle, pancreas and placenta.
    • Sequence similarities
      Belongs to the SMARCD family.
      Contains 1 SWIB domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 60 kDa BRG-1/Brm-associated factor subunit A antibody
      • 60 kDa BRG1/Brm associated factor subunit A antibody
      • BAF60A antibody
      • BRG1 associated factor 60A antibody
      • BRG1-associated factor 60A antibody
      • Chromatin remodeling complex BAF60A subunit antibody
      • CRACD1 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 60A antibody
      • Rsc6p antibody
      • SMARCD1 antibody
      • SMRD1 antibody
      • SMRD1_HUMAN antibody
      • SWI/SNF complex 60 kDa subunit A antibody
      • SWI/SNF complex 60 kDa subunit antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin d1 antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 antibody
      • Swp73 like protein antibody
      see all

    Images

    • All lanes : Anti-SMARCD1 antibody (ab81621) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg/ml
      Lane 2 : HeLa cell lysate at 15 µg/ml
      Lane 3 : HeLa cell lysate at 5 µg/ml

      Developed using the ECL technique

      Predicted band size : 58 kDa
      Observed band size : 58 kDa


      Exposure time : 30 seconds

    References

    This product has been referenced in:
    • Petrelli A  et al. By promoting cell differentiation, miR-100 sensitizes basal-like breast cancer stem cells to hormonal therapy. Oncotarget 6:2315-30 (2015). WB . Read more (PubMed: 25537513) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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