The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
FunctionInvolved in chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
Tissue specificityExpressed in all tissues tested, including brain, heart, kidney, liver, lung, muscle, pancreas and placenta.
Sequence similaritiesBelongs to the SMARCD family. Contains 1 SWIB domain.
SWI/SNF related matrix associated actin dependent regulator of chromatin d1 antibody
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 antibody
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 antibody
Swp73 like protein antibody
Anti-SMARCD1 antibody images
Western blot - SMARCD1 antibody (ab83208)
Anti-SMARCD1 antibody (ab83208) at 1 µg/ml + Human fetal liver lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 58 kDa Observed band size : 58 kDa Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
References for Anti-SMARCD1 antibody (ab83208)
This product has been referenced in:
Goljanek-Whysall K et al. myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis. Development141:3378-87 (2014).
Read more (PubMed: 25078649) »
Xu L et al. MicroRNA-7-regulated TLR9 signaling-enhanced growth and metastatic potential of human lung cancer cells by altering the phosphoinositide-3-kinase, regulatory subunit 3/Akt pathway. Mol Biol Cell24:42-55 (2013).
Read more (PubMed: 23135998) »