Overview

  • Product name
  • Description
    Rabbit polyclonal to SMARCD2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: STDPQDFIQE WLRSQRRDLK IITDVIGNPE EERRAAFYHQ PWAQEAVGRH, corresponding to amino acids 386-435 of Human SMARCD2

  • Positive control
    • Jurkat cell lysate and Human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab56241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 16 µg/ml.

Target

  • Relevance
    SMARCD2 is a member of the SWI/SNF family of proteins which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. It is part of the large ATP-dependent chromatin remodeling complex SNF/SWI.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • 60 kDa BRG 1/Brm associated factor subunit B antibody
    • 60 kDa BRG1/Brm associated factor subunit B antibody
    • BAF60B antibody
    • BRG1 associated factor 60B antibody
    • Chromatin remodeling complex BAF60B subunit antibody
    • CRACD 2 antibody
    • CRACD2 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 60B antibody
    • PRO2451 antibody
    • Rsc6p antibody
    • SMARCD 2 antibody
    • SWI/SNF complex 60 kDa subunit B antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin d2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 2 antibody
    • Swp73 like protein antibody
    see all

Images

  • Anti-SMARCD2 antibody (ab56241) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling SMARCD2 with ab56241 at 16µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

References

ab56241 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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