The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPlays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Tissue specificityIsoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
Sequence similaritiesBelongs to the SMARCD family. Contains 1 SWIB domain.
SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 3 antibody
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3, isoform 1 antibody
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 antibody
Swp73 like protein antibody
Anti-SMARCD3 antibody images
Western blot - Anti-SMARCD3 antibody (ab115917)
Anti-SMARCD3 antibody (ab115917) at 1 µg/ml + Mouse brain lysate at 10 µg
Predicted band size : 55 kDa
References for Anti-SMARCD3 antibody (ab115917)
This product has been referenced in:
Kumar S et al. Functional screening of mammalian mechanosensitive genes using Drosophila RNAi library- Smarcd3/Bap60 is a mechanosensitive pro-inflammatory gene. Sci Rep6:36461 (2016).
Read more (PubMed: 27819340) »