Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMARCD3 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary. Database link: Q6STE5
Plays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Isoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
Belongs to the SMARCD family. Contains 1 SWIB domain.
Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab171075 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-SMARCD3 antibody [EPR5538(2)] (ab171075) at 1/10 dilution
Lane 1 : Immunoprecipitation pellet from HeLa cell lysate Lane 2 : 1x PBS
Secondary All lanes : HRP-conjugated anti-rabbit IgG