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Synthetic peptide (Human) conjugated to KLH - which represents a portion within the last 100 amino acids of the human Structural Maintenance of Chromosomes-1 (GenBank PID 5453642).
Our Abpromise guarantee covers the use of ab9262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|ICC||1/200 - 1/800.|
|IP||Use a concentration of 1 - 4 µg/ml.|
|WB||1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).|
|ChIP||Use at an assay dependent dilution. PubMed: 20966046|
Interphase HeLa cells stained with ab9262 (1/500). The antibody gave nuclear staining in all interphase nuclei investigated. However, the antibody failed to recognize any chromatin-associated epitopes in prophase or metaphase cells suggesting that the epitopes may be masked during mitosis. ab9262 staining is shown in green. The cells were counterstained with DAPI (red). 100x magnification.
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