Recombinant
RabMAb

Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)

Overview

  • Product name
    Anti-SMC1 (phospho S957) antibody [EPR2857Y]
    See all SMC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2857Y] to SMC1 (phospho S957)
  • Specificity
    Detects SMC1 only when phosphorylated on serine 957.
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-peptide corresponding to residues surrounding serine 957 of human SMC1

  • Positive control
    • Hela cell lysate; human breast carcinoma or tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 143 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • CDLS2 antibody
      • DKFZp686L19178 antibody
      • DXhXs423e antibody
      • DXS423E antibody
      • KIAA0178 antibody
      • MGC138332 antibody
      • OTTHUMP00000061876 antibody
      • RP6 29D12.1 antibody
      • SB1.8 antibody
      • Segregation of mitotic chromosomes 1 antibody
      • Segregation of mitotic chromosomes like 1 antibody
      • SMC 1 antibody
      • SMC protein 1B antibody
      • SMC-1-beta antibody
      • SMC-1B antibody
      • SMC1 antibody
      • SMC1A antibody
      • SMC1alpha antibody
      • SMC1alpha protein antibody
      • SMC1B antibody
      • SMC1B_HUMAN antibody
      • SMC1BETA antibody
      • SMC1beta protein antibody
      • SMC1L1 antibody
      • SMC1L2 antibody
      • SMCB antibody
      • Structural maintenance of chromosome 1 like 1 protein antibody
      • Structural maintenance of chromosome 1 like 2 protein antibody
      • Structural maintenance of chromosomes 1A antibody
      • Structural maintenance of chromosomes 1B antibody
      • Structural maintenance of chromosomes protein 1B antibody
      see all

    Images

    • All lanes : Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768) at 1/1000 dilution

      Lane 1 : Hela cells lysate, untreated
      Lane 2 : Hela cells lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 143 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    • ab75768 at 1/100 dilution staining SMC1 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody.
    • ab75768 at 1/100 dilution staining SMC1 in human tonsil tissue, using a HRP/AP polymerized secondary antibody.

    References

    ab75768 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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