Overview

  • Product name
    Anti-SMC1 (phospho S966) antibody
    See all SMC1 primary antibodies
  • Description
    Rabbit polyclonal to SMC1 (phospho S966)
  • Tested applications
    Suitable for: IHC-P, IP, WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Orangutan
  • Immunogen

    Rabbits were immunized with a phosphorylated synthetic peptide, which represented the portion of human Structural Maintenance of Chromosomes-1 (LocusLink ID 8243)around Serine 966, conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.

  • Positive control
    • HeLa cells treated with 10 Gy IR for 1hr. IHC-P: FFPE human spleen tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab1276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-6 µg/mg of lysate.
WB 1/500 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • CDLS2 antibody
    • DKFZp686L19178 antibody
    • DXhXs423e antibody
    • DXS423E antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • OTTHUMP00000061876 antibody
    • RP6 29D12.1 antibody
    • SB1.8 antibody
    • Segregation of mitotic chromosomes 1 antibody
    • Segregation of mitotic chromosomes like 1 antibody
    • SMC 1 antibody
    • SMC protein 1B antibody
    • SMC-1-beta antibody
    • SMC-1B antibody
    • SMC1 antibody
    • SMC1A antibody
    • SMC1alpha antibody
    • SMC1alpha protein antibody
    • SMC1B antibody
    • SMC1B_HUMAN antibody
    • SMC1BETA antibody
    • SMC1beta protein antibody
    • SMC1L1 antibody
    • SMC1L2 antibody
    • SMCB antibody
    • Structural maintenance of chromosome 1 like 1 protein antibody
    • Structural maintenance of chromosome 1 like 2 protein antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes 1B antibody
    • Structural maintenance of chromosomes protein 1B antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of neocarzinostatin treated asynchronous HeLa cells labelling SMC1 (phospho S966) with ab1276 at 1/5000 (0.2µg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.
  • IHC image of SMC1 (phospho S966)  staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1276, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 143 kDa
    ab1276 at a 1:1000 dilution staining phospho SMC 1 (Ser 966) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract by Western Blot (ECL). ab1276 at a 1:1000 dilution staining phospho SMC 1 (Ser 966) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract by Western Blot (ECL).
  • SKN cells cultured on coverslips were fixed in 4% paraformaldehyde and then stained with Rabbit polyclonal to SMC1 (phospho S966), ab1276 (green) at a working dilution of 1/200. The DNA stained with DAPI is shown in red. (100x magnification).

References

This product has been referenced in:
  • Buscemi G  et al. Bimodal regulation of p21(waf1) protein as function of DNA damage levels. Cell Cycle 13:2901-12 (2014). WB . Read more (PubMed: 25486478) »
  • Cavalieri S  et al. Deep-intronic ATM mutation detected by genomic resequencing and corrected in vitro by antisense morpholino oligonucleotide (AMO). Eur J Hum Genet 21:774-8 (2013). Read more (PubMed: 23211698) »

See all 12 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. I can confirm that the immunogen used to generate ab1276 SMC1 (phospho S966) antibody represents a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
40 µg
Specification
HeLa cells
Treatment
X radiation (10g)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Mar 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (HeLa and SK-N-SH)
Specification
HeLa and SK-N-SH
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Oct 12 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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