Overview

  • Product name
  • Description
    Rabbit polyclonal to SMC2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Dog, Xenopus laevis, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to SMC2. The epitope maps to a region between residues 950 and 1000 of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592)

  • Positive control
    • Whole cell lysate from mock transfected or SMC2 transfected HEK293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab10411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 137 kDa).

Target

  • Function
    Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Sequence similarities
    Belongs to the SMC family. SMC2 subfamily.
  • Domain
    The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.
  • Cellular localization
    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP E antibody
    • CAPE antibody
    • Chromosome associated protein E antibody
    • Chromosome-associated protein E antibody
    • FLJ10093 antibody
    • hCAP E antibody
    • hCAP-E antibody
    • hCAPE antibody
    • PRO0324 antibody
    • SMC 2 antibody
    • SMC protein 2 antibody
    • SMC-2 antibody
    • SMC2 antibody
    • SMC2 L1 antibody
    • SMC2_HUMAN antibody
    • SMC2L1 antibody
    • Structural maintenance of chromosomes (SMC) family member chromosome associated protein E antibody
    • Structural maintenance of chromosomes 2 antibody
    • Structural maintenance of chromosomes 2 like 1 antibody
    • Structural maintenance of chromosomes 2 yeast like 1 antibody
    • Structural maintenance of chromosomes protein 2 antibody
    • XCAP E homolog antibody
    • XCAP-E homolog antibody
    • XCAPE homolog antibody
    see all

Images



  • Predicted band size : 137 kDa


    ab10411 detection of human SMC2 by Western blot. Samples: Whole cell lysate (90 µg) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody: ab10411 used at 0.1 µg/ml. Detection: Chemiluminescence with a 10 minute exposure.

References

ab10411 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast)
Loading amount
25 µg
Specification
fibroblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted May 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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