Synthetic peptide. The epitope maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Our Abpromise guarantee covers the use of ab10412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/5000 - 1/20000. Detects a band of approximately 140 kDa (predicted molecular weight: 137 kDa). | |
IP | Use at 2-10 µg/mg of lysate. | |
IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | 1/200 - 1/400. |
ab10412 detection of human SMC2 by Western blot and Immunoprecipitation. Samples: Whole cell lysate (90
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colorectal tissue, staining SMC2 with ab10412 at 1/200 dilution. Staining was detected using DAB.
N, normal tissue; T: tumor tissue (adenocarcinoma)Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"