• Product nameAnti-SMC3 antibody
    See all SMC3 primary antibodies
  • Description
    Rabbit polyclonal to SMC3
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1175 and C-terminal (residue 1217) of Human SMC3 (NP_005436.1)

  • Positive control
    • Human ovarian carcinoma or Mouse squamous cell carcinoma tissue.



Our Abpromise guarantee covers the use of ab82551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: 1/100 - 1/500.

    Epitope exposure with citrate buffer will enhance staining and is recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCentral component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
    • Involvement in diseaseDefects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
    • Sequence similaritiesBelongs to the SMC family. SMC3 subfamily.
    • DomainThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
    • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAM antibody
      • Bamacan antibody
      • Basement membrane associated chondroitin proteoglycan antibody
      • Basement membrane-associated chondroitin proteoglycan antibody
      • BMH antibody
      • CDLS3 antibody
      • chondroitin sulfate proteoglycan 6 (bamacan) antibody
      • Chondroitin sulfate proteoglycan 6 antibody
      • Chromosome associated polypeptide antibody
      • Chromosome-associated polypeptide antibody
      • CSPG 6 antibody
      • CSPG6 antibody
      • hCAP antibody
      • Human chromosome associated polypeptide antibody
      • im:7142991 antibody
      • SMC 3 antibody
      • SMC protein 3 antibody
      • SMC-3 antibody
      • smc3 antibody
      • SMC3_HUMAN antibody
      • SMC3L1 antibody
      • Structural maintenance of chromosome 3 antibody
      • Structural maintenance of chromosomes 3 antibody
      • Structural maintenance of chromosomes protein 3 antibody
      • u:fb22e01 antibody
      • wu:fc30d07 antibody
      see all

    Anti-SMC3 antibody images

    • ab82551, at a 1:250 dilution, staining Mouse SMC3 in squamous cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
    • ab82551, at a 1:250 dilution, staining Human SMC3 in ovarian carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    References for Anti-SMC3 antibody (ab82551)

    ab82551 has not yet been referenced specifically in any publications.

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