Overview

  • Product name
    Anti-SMC3 antibody - C-terminal
    See all SMC3 primary antibodies
  • Description
    Rabbit polyclonal to SMC3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 909-1199 of Human SMC3 (UniProt Q9UQE7).

  • Positive control
    • HepG2, NIH3T3, JC, BCL-1 and PC12 whole cell lysates; HeLa cells; Human Cal27 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 141 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
  • Involvement in disease
    Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
  • Sequence similarities
    Belongs to the SMC family. SMC3 subfamily.
  • Domain
    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
  • Cellular localization
    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAM antibody
    • Bamacan antibody
    • Basement membrane associated chondroitin proteoglycan antibody
    • Basement membrane-associated chondroitin proteoglycan antibody
    • BMH antibody
    • CDLS3 antibody
    • chondroitin sulfate proteoglycan 6 (bamacan) antibody
    • Chondroitin sulfate proteoglycan 6 antibody
    • Chromosome associated polypeptide antibody
    • Chromosome-associated polypeptide antibody
    • CSPG 6 antibody
    • CSPG6 antibody
    • hCAP antibody
    • Human chromosome associated polypeptide antibody
    • im:7142991 antibody
    • SMC 3 antibody
    • SMC protein 3 antibody
    • SMC-3 antibody
    • smc3 antibody
    • SMC3_HUMAN antibody
    • SMC3L1 antibody
    • Structural maintenance of chromosome 3 antibody
    • Structural maintenance of chromosomes 3 antibody
    • Structural maintenance of chromosomes protein 3 antibody
    • u:fb22e01 antibody
    • wu:fc30d07 antibody
    see all

Images

  • Anti-SMC3 antibody - C-terminal (ab155587) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 141 kDa



    5% SDS PAGE
  • All lanes : Anti-SMC3 antibody - C-terminal (ab155587) at 1/1000 dilution

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 141 kDa



    5% SDS PAGE
  • Anti-SMC3 antibody - C-terminal (ab155587) at 1/2000 dilution + PC12 whole cell lysate at 30 µg

    Predicted band size: 141 kDa



    5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SMC3 with ab155587 at 1/200 dilution. Lower panel merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue labeling SMC3 with ab155587 at 1/100 dilution.

References

ab155587 has not yet been referenced specifically in any publications.

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