Anti-SMC3 (phospho S1083) antibody (ab83970)
Key features and details
- Rabbit polyclonal to SMC3 (phospho S1083)
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SMC3 (phospho S1083) antibody
See all SMC3 primary antibodies -
Description
Rabbit polyclonal to SMC3 (phospho S1083) -
Host species
Rabbit -
Specificity
Recognises a region surrounding residue 1083 of human SMC3, when Serine 1083 is phosphorylated, using the numbering given in SwissProt entry Q9UQE7 (GeneID 9126). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide within Human SMC3 (phospho S1083). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UQE7 -
Positive control
- human colon adenocarcinoma and mouse squamous cell carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Phospho SMC3 (S1083) immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.
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Notes |
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IHC-P
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. |
Target
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Function
Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. -
Involvement in disease
Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation. -
Sequence similarities
Belongs to the SMC family. SMC3 subfamily. -
Domain
The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. - Information by UniProt
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Database links
- Entrez Gene: 395188 Chicken
- Entrez Gene: 281729 Cow
- Entrez Gene: 9126 Human
- Entrez Gene: 13006 Mouse
- Entrez Gene: 29486 Rat
- Omim: 606062 Human
- SwissProt: O97594 Cow
- SwissProt: Q9UQE7 Human
see all -
Alternative names
- BAM antibody
- Bamacan antibody
- Basement membrane associated chondroitin proteoglycan antibody
see all
Images
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ab83970 at a 1/250 dilution, staining SMC3 (phospho 1083) in formaldehyde fixed, paraffin embedded human colon adenocarcinoma sections by immunohistochemistry. Left: Mock phosphatase treated section. Right: calf intestinal phosphatase-treated section. Detection by DAB staining.
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ab83970 at a 1/250 dilution, staining SMC3 (phospho 1083) in formaldehyde fixed, paraffin embedded mouse squamous cell carcinoma sections by immunohistochemistry. Left: Mock phosphatase treated section. Right: calf intestinal phosphatase-treated section.
Detection by DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab83970 has not yet been referenced specifically in any publications.