Anti-SMC3 (phospho S1083) antibody (ab83970)

Overview

  • Product name
    Anti-SMC3 (phospho S1083) antibody
    See all SMC3 primary antibodies
  • Description
    Rabbit polyclonal to SMC3 (phospho S1083)
  • Specificity
    Recognises a region surrounding residue 1083 of human SMC3, when Serine 1083 is phosphorylated, using the numbering given in SwissProt entry Q9UQE7 (GeneID 9126).
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • human colon adenocarcinoma and mouse squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab83970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.

Target

  • Function
    Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
  • Involvement in disease
    Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
  • Sequence similarities
    Belongs to the SMC family. SMC3 subfamily.
  • Domain
    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
  • Cellular localization
    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAM antibody
    • Bamacan antibody
    • Basement membrane associated chondroitin proteoglycan antibody
    • Basement membrane-associated chondroitin proteoglycan antibody
    • BMH antibody
    • CDLS3 antibody
    • chondroitin sulfate proteoglycan 6 (bamacan) antibody
    • Chondroitin sulfate proteoglycan 6 antibody
    • Chromosome associated polypeptide antibody
    • Chromosome-associated polypeptide antibody
    • CSPG 6 antibody
    • CSPG6 antibody
    • hCAP antibody
    • Human chromosome associated polypeptide antibody
    • im:7142991 antibody
    • SMC 3 antibody
    • SMC protein 3 antibody
    • SMC-3 antibody
    • smc3 antibody
    • SMC3_HUMAN antibody
    • SMC3L1 antibody
    • Structural maintenance of chromosome 3 antibody
    • Structural maintenance of chromosomes 3 antibody
    • Structural maintenance of chromosomes protein 3 antibody
    • u:fb22e01 antibody
    • wu:fc30d07 antibody
    see all

Images

  • ab83970 at a 1/250 dilution, staining SMC3 (phospho 1083) in formaldehyde fixed, paraffin embedded human colon adenocarcinoma sections by immunohistochemistry. Left: Mock phosphatase treated section. Right: calf intestinal phosphatase-treated section. Detection by DAB staining.
  • ab83970 at a 1/250 dilution, staining SMC3 (phospho 1083) in formaldehyde fixed, paraffin embedded mouse squamous cell carcinoma sections by immunohistochemistry. Left: Mock phosphatase treated section. Right: calf intestinal phosphatase-treated section.
    Detection by DAB staining.

References

ab83970 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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