Overview

  • Product nameAnti-SMC4 antibody
    See all SMC4 primary antibodies
  • Description
    Rabbit polyclonal to SMC4
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide, which represented a portion of human structural maintenance of chromosomes 4 encoded within exons 11 and 12.

  • Positive control
    • SMC4 transfected 239T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab17957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa).
ICC/IF 1/500 - 1/2000.

Target

  • FunctionCentral component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Tissue specificityWidely expressed. Higher expression in testis, colon, thymus.
  • Sequence similaritiesBelongs to the SMC family. SMC4 subfamily.
  • DomainThe hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
  • Cellular localizationNucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP C antibody
    • CAPC antibody
    • Chromosome associated polypeptide C antibody
    • Chromosome-associated polypeptide C antibody
    • hCAP C antibody
    • hCAP-C antibody
    • SMC 4 antibody
    • SMC protein 4 antibody
    • SMC-4 antibody
    • SMC4 antibody
    • SMC4_HUMAN antibody
    • SMC4L1 antibody
    • Structural maintenance of chromosomes 4 antibody
    • Structural maintenance of chromosomes 4 like 1 antibody
    • Structural maintenance of chromosomes protein 4 antibody
    • XCAP C homolog antibody
    • XCAP-C homolog antibody
    see all

Anti-SMC4 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SMC4 with ab17957 at 5µg/ml. Cells were fixed with methanol/actetone and blocked with 3% BSA in PBS. A Rhodamine-conjugated anti-rabbit IgG was used as the secondary antibody. Cells were counterstained with DAPI.


  • Predicted band size : 147 kDa

    Samples: Whole cell lysate (90 µg) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibodies: ab17957 used at a dilution of 1/5,000 and goat anti-rabbit HRP used at a dilution of 1:20,000. Detection: Chemiluminescence with 5 second exposure.

    Samples: Whole cell lysate (90 µg) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibodies: ab17957 used at a dilution of 1/5,000 and goat anti-rabbit HRP used at a dilution of 1:20,000. Detection: Chemiluminescence with 5 second exposure.

References for Anti-SMC4 antibody (ab17957)

ab17957 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Nov 02 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"