The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 129 kDa).
Use a concentration of 5 - 10 µg/ml.
Application notesIs unsuitable for ICC/IF.
RelevanceStructural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics.
Cellular localizationNucleus. Chromosome. Chromosome; telomere. Note: Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
SMC 5 structural maintenance of chromosomes 5 like 1 antibody
SMC protein 5 antibody
SMC5 L1 antibody
SMC5 protein antibody
SMC5 structural maintenance of chromosomes 5 like 1 antibody
Structural maintenance of chromosomes 5 antibody
Structural maintenance of chromosomes 5 like 1 antibody
Structural maintenance of chromosomes protein 5 antibody
Anti-SMC5 antibody images
Western blot - SMC5 antibody (ab18038)
Predicted band size : 129 kDa
Ab18038 staining Human SMC5 at a concentration of 0.1 µg/ml by Western Blot, and 5 µg/0.5 mg lysate by Immunoprecipitation.
A) Nuclear extract (10 and 20 µg) from HeLa cells.
B) Whole cell lysate (500 µg) from HeLa and 293T cells.
Detection: Chemiluminescence with exposure times of less than 2 minutes.
References for Anti-SMC5 antibody (ab18038)
This product has been referenced in:
Gallego-Paez LM et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell25:302-17 (2014).
Read more (PubMed: 24258023) »