• Product nameAnti-SMC5 antibody
    See all SMC5 primary antibodies
  • Description
    Rabbit polyclonal to SMC5
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 1050-1101 of Human SMC5, using the numbering given in TrEMBL entry Q8IY18 (GeneID 23137).

  • Positive control
    • Nuclear extract from HeLa cells Whole cell lysate from HeLa and 293T cells



Our Abpromise guarantee covers the use of ab18038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 129 kDa).
IP Use a concentration of 5 - 10 µg/ml.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • RelevanceStructural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics.
    • Cellular localizationNucleus. Chromosome. Chromosome; telomere. Note: Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
    • Database links
    • Alternative names
      • hSMC 5 antibody
      • hSMC5 antibody
      • KIAA0594 antibody
      • MLZ-453 antibody
      • OTTHUMP00000063367 antibody
      • SMC 5 antibody
      • SMC 5 structural maintenance of chromosomes 5 like 1 antibody
      • SMC protein 5 antibody
      • SMC5 L1 antibody
      • SMC5 protein antibody
      • SMC5 structural maintenance of chromosomes 5 like 1 antibody
      • SMC5L1 antibody
      • Structural maintenance of chromosomes 5 antibody
      • Structural maintenance of chromosomes 5 like 1 antibody
      • Structural maintenance of chromosomes protein 5 antibody
      see all

    Anti-SMC5 antibody images

    • Predicted band size : 129 kDa

      Ab18038 staining Human SMC5 at a concentration of 0.1 µg/ml by Western Blot, and 5 µg/0.5 mg lysate by Immunoprecipitation.


      A) Nuclear extract (10 and 20 µg) from HeLa cells.

      B) Whole cell lysate (500 µg) from HeLa and 293T cells.

      Detection: Chemiluminescence with exposure times of less than 2 minutes.

    References for Anti-SMC5 antibody (ab18038)

    This product has been referenced in:
    • Gallego-Paez LM  et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell 25:302-17 (2014). Human . Read more (PubMed: 24258023) »
    • Li J  et al. Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas. Neuro Oncol 10:45-51 (2008). Read more (PubMed: 18182627) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TritonX100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 02 2007