Overview

  • Product name
  • Description
    Rabbit polyclonal to SMC6L1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1050 and the C-terminus (residue 1091) of Human SMC6L1 (Swiss-Prot Q96SB8)

  • Positive control
    • Human ovarian and breast tumor tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab86103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies.
    • Tissue specificity
      Widely expressed. Overexpressed in testis.
    • Sequence similarities
      Belongs to the SMC family. SMC6 subfamily.
    • Domain
      The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC5, forming a V-shaped heterodimer.
    • Post-translational
      modifications
      Sumoylated by NSMCE2/MMS21.
      Ubiquitinated.
    • Cellular localization
      Nucleus. Chromosome. Chromosome > telomere. Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ22116 antibody
      • FLJ35534 antibody
      • hSMC6 antibody
      • SMC 6 antibody
      • SMC protein 6 antibody
      • SMC-6 antibody
      • SMC6 antibody
      • SMC6 protein antibody
      • SMC6 structural maintenance of chromosomes 6 like 1 antibody
      • SMC6_HUMAN antibody
      • Smc6l1 antibody
      • Structural maintenance of chromosomes 6 antibody
      • Structural maintenance of chromosomes 6 like 1 antibody
      • Structural maintenance of chromosomes protein 6 antibody
      see all

    Anti-SMC6L1 antibody images

    • ab86103, at 1/250 dilution, staining SMC6L1 in formalin-fixed, paraffin-embedded human ovarian tumor tissue by Immunohistochemistry using DAB staining.
    • ab86103, at 1/250 dilution, staining SMC6L1 in formalin-fixed, paraffin-embedded human breast tumor tissue by Immunohistochemistry using DAB staining.

    References for Anti-SMC6L1 antibody (ab86103)

    ab86103 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 15 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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