Overview

  • Product nameAnti-SMCHD1 antibody
    See all SMCHD1 primary antibodies
  • Description
    Rabbit polyclonal to SMCHD1
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: DNGRGMTSKQ LNNWAVYRLS KFTRQGDFES DHSGYVRPVP VPRSLNSDIS YFGVGGKQAV FFVGQSARMI SKPADSQDVH ELVLSKED, corresponding to N terminal amino acids 184-271 of Human SMCHD1.

  • Positive control
    • U 251 MG cell lysates; Human testis tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-SMCHD1 antibody images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122555 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122555, at 1/50 dilution, staining SMCHD1 in Paraffin Embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-SMCHD1 antibody (ab122555) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

References for Anti-SMCHD1 antibody (ab122555)

This product has been referenced in:
  • Sunwoo H  et al. The Xist RNA-PRC2 complex at 20-nm resolution reveals a low Xist stoichiometry and suggests a hit-and-run mechanism in mouse cells. Proc Natl Acad Sci U S A 112:E4216-25 (2015). Mouse . Read more (PubMed: 26195790) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (ES cells)
Specification ES cells
Permeabilization Yes - 0.5% TritonX-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 20 2013

Application Western blot
Sample Mouse Cell lysate - whole cell (fibroblast)
Loading amount 50 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2013

I am very pleased to hear you would like to test ab31865, ab122555, ab136973, and ab154105 in mouse. These codes will give you the value listed above off future orders before the expiration date. To redeem this offer, please submit an Abreview for mous...

Read More

Thank you very much for your interest our products.

To our knowledge, ab31865 and ab122555 have not been tested in mouse. Since we carry over 100,000 products, we are unable to store multiple vial sizes for samples. However by participating ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"