Overview

  • Product name
  • Description
    Rabbit polyclonal to SMCHD1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: DNGRGMTSKQ LNNWAVYRLS KFTRQGDFES DHSGYVRPVP VPRSLNSDIS YFGVGGKQAV FFVGQSARMI SKPADSQDVH ELVLSKED, corresponding to N terminal amino acids 184-271 of Human SMCHD1.

  • Positive control
    • U 251 MG cell lysates; Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • BAMS antibody
    • FSHD2 antibody
    • KIAA0650 antibody
    • SMC hinge domain containing 1 antibody
    • SMC hinge domain containing protein 1 antibody
    • Smchd1 antibody
    • SMHD1_HUMAN antibody
    • Structural maintenance of chromosomes flexible hinge domain containing 1 antibody
    • Structural maintenance of chromosomes flexible hinge domain-containing protein 1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122555 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122555, at 1/50 dilution, staining SMCHD1 in Paraffin Embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-SMCHD1 antibody (ab122555) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

References

This product has been referenced in:
  • Shaw ND  et al. SMCHD1 mutations associated with a rare muscular dystrophy can also cause isolated arhinia and Bosma arhinia microphthalmia syndrome. Nat Genet 49:238-248 (2017). Read more (PubMed: 28067909) »
  • Sunwoo H  et al. The Xist RNA-PRC2 complex at 20-nm resolution reveals a low Xist stoichiometry and suggests a hit-and-run mechanism in mouse cells. Proc Natl Acad Sci U S A 112:E4216-25 (2015). Mouse . Read more (PubMed: 26195790) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (ES cells)
Specification
ES cells
Permeabilization
Yes - 0.5% TritonX-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 20 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast)
Loading amount
50 µg
Specification
fibroblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2013

I am very pleased to hear you would like to test ab31865, ab122555, ab136973, and ab154105 in mouse. These codes will give you the value listed above off future orders before the expiration date. To redeem this offer, please submit an Abreview for mous...

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Thank you very much for your interest our products.

To our knowledge, ab31865 and ab122555 have not been tested in mouse. Since we carry over 100,000 products, we are unable to store multiple vial sizes for samples. However by participating ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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