antigen sequence: DNGRGMTSKQ LNNWAVYRLS KFTRQGDFES DHSGYVRPVP VPRSLNSDIS YFGVGGKQAV FFVGQSARMI SKPADSQDVH ELVLSKED, corresponding to N terminal amino acids 184-271 of Human SMCHD1.
Our Abpromise guarantee covers the use of ab122555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment