Overview

  • Product nameAnti-SMCHD1 antibody
    See all SMCHD1 primary antibodies
  • Description
    Rabbit polyclonal to SMCHD1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1750 - 1850 of Human SMCHD1.

    (Peptide available as ab31864.)

  • Positive control
    • This antibody gave a positive signal for Immunofluorescence in the following cell lines; HEK293, HeLa, MCF7, HepG2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 21836164
ICC/IF 1/100.

Target

Anti-SMCHD1 antibody images

  •  ab31865 (1/100) staining SMCHD1 by immunofluorescence in assynchronous HeLa cells (green). Secondary antibody: goat polyclonal antibody conjugated to Cy3; Counterstain: DAPI (red). Please refer to Abreview for further information.

    See Abreview

  • ICC/IF image of ab31865 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31865, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methaol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

References for Anti-SMCHD1 antibody (ab31865)

This product has been referenced in:
  • Brideau NJ  et al. Independent Mechanisms Target SMCHD1 to Trimethylated Histone H3 Lysine 9-Modified Chromatin and the Inactive X Chromosome. Mol Cell Biol 35:4053-68 (2015). Read more (PubMed: 26391951) »
  • Massah S  et al. Epigenetic characterization of the growth hormone gene identifies SmcHD1 as a regulator of autosomal gene clusters. PLoS One 9:e97535 (2014). WB ; Mouse . Read more (PubMed: 24818964) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 50 µg
Specification MEF
Gel Running Conditions Reduced Denaturing (7%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Feb 20 2013

I am very pleased to hear you would like to test ab31865, ab122555, ab136973, and ab154105 in mouse. These codes will give you the value listed above off future orders before the expiration date. To redeem this offer, please submit an Abreview for mous...

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Thank you very much for your interest our products.

To our knowledge, ab31865 and ab122555 have not been tested in mouse. Since we carry over 100,000 products, we are unable to store multiple vial sizes for samples. However by participating ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"