The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 13 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in sperm motility. Its absence is associated with genetic background dependent male infertility. Infertility may be due to reduced sperm motility in the female reproductive tract and inability to penetrate the oocyte zona pellucida.
Tissue specificityTestis. Is selectively expressed in the spermatids of seminiferous tubules.
Developmental stageExpressed in postmeiotic cells.
Cellular localizationCytoplasm. Mitochondrion membrane. Becomes associated with the spermatid mitochondrion capsule at step 16 of spermatogenesis.